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Image Search Results
Journal: Scientific Reports
Article Title: Caerin 1.1 and 1.9 peptides induce acute caspase 3/GSDME-mediated pyroptosis in epithelial cancer cells
doi: 10.1038/s41598-025-96438-0
Figure Lengend Snippet: F1/F3 mediates HeLa cell pyroptosis through the caspase-3/GSDME signalling pathway. ( A )-( I ): HeLa cells (1.0 × 10 6 ) were treated with F1/F3 (10 µg/ml), P3 or left untreated for 1 h, the expression of pyroptosis-related proteins was tested by Western blot. ( A ) GSDMD. ( B ) Caspase-1. ( C ) Caspase-4. ( D ) Caspase-5. ( E ) Cleaved caspase-4. ( F ) GSDMB. ( G ) Caspase-3. ( H ) Cleaved caspase-3. ( I ) Cleaved GSDME-N. ( J ) LDH release was detected by F1/F3, caspase inhibitor and caspase-3 inhibitor (Ac-DEVD-CHO) combined with F1/F3. These results come from a representative experiment in which two independent experiments were conducted. All data are presented as the mean ± SEM. * P -value < 0.05, ** P -value < 0.01, *** P -value < 0.001. The statistical significance was assessed using one-way ANOVA.
Article Snippet:
Techniques: Expressing, Western Blot